Tritc-phalloidin
WebPhalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin in … WebThey were stained with phalloidin-TRITC (A–C) and anti- β-integrin antibodies (D–F) and visualized by fluorescence confocal microscopy. S2R+ cells plated on glass shrank around their nuclei showing extensive actin-rich lamellipodia and occasional short filopodia and punctate β-integrin expression at the margins (arrowhead) and dispersed ...
Tritc-phalloidin
Did you know?
WebSep 17, 2024 · We visualised effects on the cytoskeleton and nucleus using TRITC-phalloidin and DAPI staining respectively. The wild type Pnf protein had a very clear effect on the cells, producing phenotypes consistent with those predicted by similarity to the CNF2 toxin. CNF2 is known to modify the cellular Rho GTPases, RhoA, Rac1 and Cdc42. WebTypically, it is used conjugated to a fluorescent dye, such as FITC, Rhodamine, TRITC or similar dyes, such as Alexa Fluor ® 488 or iFluor 488. Phalloidin can be used with sample types such as formaldehyde-fixed and permeabilized tissue sections, cell cultures and cell-free experiments.
WebMar 22, 2024 · Stain with 100 μl TRITC-labeled phalloidin staining solution/well on a 96-well plate overnight at 4°C. 8. Make a window (~5 mm squares) in the center of a piece of plastic tape that is slightly larger than a cover glass on a microscope slide and attach the tape to the microscope slide (Fig. 1g). 9. WebPhalloidin is a highly selective bicyclic peptide that is used for staining actin filaments (also known as F-actin). It binds to all variants of actin filaments in many different species of …
WebAfter incubation, the cells were fixed with 4% paraformaldehyde for 20 min at RT, then treated with 0.5% Triton X-100 solution. The nuclei and cytoskeleton were then stained with DAPI and TRITC-phalloidin (Solarbio, China), respectively. Images were recorded using an inverted fluorescence microscope (Nikon A1 MP, Japan). WebPhalloidin conjugated to TRITC via the side chain of amino acid 7. The conjugate has a molecular weight of 1300. This fluorochrome has an excitation maximum of 540 nm and …
WebPhalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals.
WebTRITC-phalloidin (P1951) and p -phenylenediamine (P6001) are from Sigma-Aldrich (St. Louis, MO). C3 toxin (BML-G130) is from Enzo Life Science (Plymouth Meeting, PA) or can be purified from Escherichia coli transformed with a plasmid encoding a glutathione S-transferase/C3 toxin fusion protein ( Pierce et al., 1999 ). 1. footwear logo with wingsWebTetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications. elim christian centre blenheimWebDescription The VECTASHIELD HardSet Antifade Mounting Medium with TRITC-Phalloidin prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. Fluorescent … elim church aberystwythWebMar 26, 2024 · After ANGII induction, cells were fixed in 4% paraformaldehyde for 10 min, stimulated by 0.1% TritonX-100 for 5 min, and stained with TRITC Phalloidin solution (1 μg/mL) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, 10 μg/mL) for 1 h. Cells were rinsed with phosphate buffer solution (PBS) three times between every two steps … footwear logo makerWebThe TRITC-phalloidin stain- ing results of actin filaments of MDA-MB-231 cells seeded on flat surfaces and treated with various con- centrations of emodin for 2 h are given in Fig. 2. footwear logo ideasWebMar 6, 2024 · 爱必信(absin)特色优质生物试剂产品有:WB相关,IHC相关,激动剂抑制剂,血清,凋亡试剂盒,Elisa试剂盒,抗体,重组蛋白等生化试剂 ... footwear machinery fairs taiwanWebMar 1, 2024 · Phalloidin-TRITC is used at a concentration of 15 μg/mL and DAPI is used at a concentration of 0.5 μg/mL. These dilutions are purely indicative and must be adapted to each brand of antibody used and to each experimental condition (sample size, exposure time, type of microscope used), possibly after a titration phase. footwear logo design ideas